| wwc1

Protein KIBRA also known as kidney and brain expressed protein (KIBRA) or WW domain-containing protein 1 (WWC1) is a protein that is encoded by the WWC1 gene.A single nucleotide polymorphism (rs17070145) in the gene has been associated with human memory performance and cognitive ability in various studies since 2006. While no significant support for KIBRA's association with memory was found in a 2008 study with 584 subjects, the original 2006 study was replicated in a smaller sample of an elderly population in 2008.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	2.64	1.78	1.09	0.64	0.41	0.31	0.23	0.09
Corrected OD	2.55	1.69	1	0.55	0.32	0.22	0.14	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse wwc1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	86-101	96
Cell Culture Supernates(n=8)	92-105	103

 

| Linearity

Samples were spiked with high concentrations of Mouse wwc1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	86-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	95	98